“When we have a lead, we just defend too much,” Larkin said.Īrizona has five points in its last three games, starting with a 3-2 victory in St. Lucas Raymond, who assisted on Larkin’s goal, is among the league leaders among rookies with 12 assists. Larkin has five goals in his last five games and six in his last seven. You can’t leave things to chance.”ĭylan Larkin scored his ninth goal of the season for the Detroit Red Wings, who went winless on a four-game trip. In the end, we had breakdown that led to that. “They reviewed it and they felt differently.
“We thought it was goalie interference, but that’s the way it goes," Detroit coach Jeff Blashill said. The Red Wings disputed Keller's goal, which was reviewed for several minutes before being allowed. “We had the puck a lot in the third so I thought I could have ended it before then, but definitely feels good to see that one go in at the end.” “I thought we played played hard,” Keller said. Keller's winning goal came when he picked up a loose puck on the right side of the net after if bounced through a crowd and fired it into an open net. Ryan Dzingel scored his second goal in as many games for Arizona, and Scott Wedgewood had 33 saves - two days after tying a career high with 42 saves in a 5-4 shootout loss to Columbus. The green bar charts to the right of the heat maps show the distribution of methylation levels, projected onto the y-axis of the heat maps.GLENDALE, Ariz, - Clayton Keller scored 25 seconds into overtime to give the Arizona Coyotes a 2-1 win over the Detroit Red Wings on Saturday night. The blue bar charts above each heat map show the distribution of CG densities, projected onto the x-axis of the heat maps. The red gradient hypo indicates the abundance of CGs that fall into bins of given methylation levels and CG densities. The thin black lines within each heat map denote the median methylation level of CGs at the given local density.
Methylation level ( y-axis) is defined as the mean methylation level of cytosines in CGs. CG density ( x-axis) is defined as the number of CG dinucleotides in 200 bp windows. Each panel represents a separate feature, and n refers to the number of analyzed CGs (per-strand depth ≥10) within that feature. Heat maps show distinct methylation and CG density patterns for different genomic features in WT ( A) and Tg( fabp10:nras 61K) transgenic zebrafish ( B). Landscape of methylome in WT and Tg( fabp10:nras 61K) transgenic zebrafish (12 mpf). This work indicates that our transgenic zebrafish could be a valuable model, not only for studying liver cancer, but also for exploring new therapeutic targets. In summary, our study provides a comprehensive analysis of molecular mechanisms accompanying the progressive nras 61K -induced ICC. Moreover, three potential biomarker genes were hypomethylated in the tumorigenesis of ICC: ehf, epha4 and itgb6.
In particular, our result discovered eleven potential biomarker genes of ICC which were conserved between zebrafish and humans. Analysis of DNA methylomes revealed that compared with WT samples, methylated genes in ICC samples were enriched in functions associated with cellular, single-organism and metabolic processes. Transcriptome analysis and gene set enrichment analysis (GSEA) of liver samples of the ICC and WT (wild-type) zebrafish revealed that the genes differentially expressed between the two groups were mainly involved in focal adhesion, chemokine signaling and metabolic pathways. In this study, we successfully constructed an nras 61K -induced ICC model in zebrafish. Intrahepatic cholangiocarcinoma (ICC) is a highly malignant tumor, difficult to diagnose even at an early stage.
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